Michael R. Armbruster et al. “Online buffer exchange coupled to ion mobility-mass spectrometry for the rapid, automated analysis of antibodies from formulation.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007424/v2

Online-buffer-exchange cleanup and fragmentation-performance results were not retained

NISTmAb in 15 mM sodium chloride and 200 mM ammonium acetate after online buffer exchange
IM-MS Compact, monomodal, native-like ion population and charge-state distribution
Main, p. 5
NISTmAb in 200 mM ammonium acetate and 1× Rituxan formulation buffer after heat stress at 55 °C for two hours
CIU NISTmAb stressed in ammonium acetate produced a higher CIU₅₀-1 than NISTmAb in 1× Rituxan formulation buffer
Main, p. 7; SI, p. S7
Crude Rituxan–DBCO-PEG₃-CHO reaction mixture after online buffer exchange
Native MS, deconvoluted mass Average DAR 1.81 ± 0.22; individual drug-bound states were not resolved because of the TOF resolution and interfering glycosylation peaks
Main, p. 7
Separate Rituxan–DBCO-PEG₃-CHO reaction after offline desalting
High-resolution Orbitrap MS (static nESI) Resolved glycosylation peaks for each DAR; average DAR 1.62