| Circular dichroism, approximately 6 μM G4 DNA, 20 % (w/v) EG or PEG, approximately 25 °C | The spectra in the absence and presence of EG or PEG were very similar; the signature peaks at 265 and 240 nm remained completely stable |
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Ajay Kumar Chand, Pramod Kumar, Harsh Sahu, Shubham Kumar Sharma, Sobhan Sen. “Interplay of depletion-interaction and viscosity drives non-monotonic ligand binding to DNA G-quadruplex under crowding.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007676/v1
Values presented only in the circular-dichroism, fluorescence, and fluorescence-correlation plots and in the image-based table in Figure 2 were not transcribed
| Steady-state fluorescence, approximately 10 nM G4 DNA, TMPyP4 up to 1.5 μM | K approximately 27.63 × 10⁶ M⁻¹ in buffer, approximately 6.26 × 10⁶ M⁻¹ in 20 % (w/v) PEG 6K, and approximately 18.00 × 10⁶ M⁻¹ in 20 % (w/v) PEG 35K |
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| Fluorescence correlation spectroscopy, approximately 1 nM G4 DNA, TMPyP4 up to 500 nM | kₒₙ 5.53 (±0.51) × 10⁸ M⁻¹ s⁻¹ in 20 % (w/v) PEG 10K and 7.09 (±0.78) × 10⁸ M⁻¹ s⁻¹ in 20 % (w/v) PEG 35K |
| Fluorescence correlation spectroscopy, TMPyP4 in buffer | Diffusion time 41.11 (±1.20) μs; hydrodynamic radius 0.65 (±0.02) × 10⁻⁹ m |
| Fluorescence correlation spectroscopy, buffer | Q 0.05; Rh6G diffusion time 37.46 (±0.80) μs; η 0.89 (±0.05) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 143.57 (±4.30) μs; G4 hydrodynamic radius 2.27 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 377.24 (±25.80) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 104.21 (±5.80) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) EG | Q 0.25; Rh6G diffusion time 67.57 (±1.93) μs; η 1.61 (±0.08) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 239.09 (±4.30) μs; G4 hydrodynamic radius 2.09 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 209.15 (±15.90) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 62.58 (±4.00) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 200 | Q 0.51; Rh6G diffusion time 85.93 (±2.39) μs; η 2.04 (±0.11) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 268.28 (±5.40) μs; G4 hydrodynamic radius 1.85 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 164.46 (±14.10) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 55.77 (±2.70) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 400 | Q 0.39; Rh6G diffusion time 100.27 (±2.26) μs; η 2.38 (±0.10) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 333.87 (±4.20) μs; G4 hydrodynamic radius 1.97 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 140.93 (±21.20) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 44.81 (±2.50) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 4K | Q 0.27; Rh6G diffusion time 171.18 (±3.03) μs; η 4.10 (±0.14) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 557.95 (±6.00) μs; G4 hydrodynamic radius 1.92 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 82.55 (±16.60) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 26.82 (±1.80) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 6K | Q 0.32; Rh6G diffusion time 243.37 (±3.99) μs; η 5.78 (±0.19) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 782.42 (±5.10) μs; G4 hydrodynamic radius 1.90 (±0.18) × 10⁻⁹ m; TMPyP4 diffusion coefficient 58.07 (±10.90) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 19.12 (±1.00) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 10K | Q 0.31; Rh6G diffusion time 267.50 (±3.68) μs; η 6.35 (±0.37) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 890.70 (±14.50) μs; G4 hydrodynamic radius 1.97 (±0.19) × 10⁻⁹ m; TMPyP4 diffusion coefficient 52.83 (±10.00) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 16.80 (±0.60) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 20K | Q 0.24; Rh6G diffusion time 330.99 (±4.16) μs; η 7.86 (±0.23) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 1076.30 (±7.60) μs; G4 hydrodynamic radius 1.93 (±0.20) × 10⁻⁹ m; TMPyP4 diffusion coefficient 42.70 (±11.40) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 13.90 (±1.00) × 10⁻¹² m² s⁻¹ |
| Fluorescence correlation spectroscopy, 20 % (w/v) PEG 35K | Q 0.29; Rh6G diffusion time 354.83 (±4.73) μs; η 8.43 (±0.40) × 10⁻³ Pa s; 5′-Cy3-G4 diffusion time 1250.00 (±16.30) μs; G4 hydrodynamic radius 2.09 (±0.19) × 10⁻⁹ m; TMPyP4 diffusion coefficient 39.83 (±21.70) × 10⁻¹² m² s⁻¹; G4 diffusion coefficient 11.97 (±0.50) × 10⁻¹² m² s⁻¹ |