Helena Roberts-Mataric, Mark Walker, Alanna Schepartz. “Novel RiPP cyclodehydratases from thermophilic bacteria edit folded polypeptides.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15002766/v2

Mass-spectrometry values presented only in plots or images were not transcribed

MicD, TteD and TcuD
Circular dichroism, 1 mg/mL in 10 mM potassium phosphate, 100 mM NaCl, pH 7.0, 25 °C, 200–300 nm Generally similar profiles; TteD and TcuD were particularly similar to each other
Circular-dichroism thermal melt, 222 nm, 20–95 °C MicD: Tₘ,app1 35.9 °C, 95 % CI [34.9, 37.0], Tₘ,app2 68.7 °C, 95 % CI [68.3 to 69.1]; TteD: Tₘ,app1 41.3 °C, 95 % CI [40.9 to 41.8], Tₘ,app2 63.9 °C, 95 % CI [58.1 to 66.4]; TcuD: Tₘ,app1 43.9 °C, 95 % CI [43.3 to 44.5], Tₘ,app2 52.6 °C, 95 % CI [42.5 to 55.5]
Differential scanning fluorimetry with SYPRO thermal shift dye, 5 µM in 10 mM potassium phosphate, 100 mM NaCl, pH 7.0, 5–95 °C MicD: Tₘ,app1 37.9 °C, 95 % CI [37.8, 38.0], Tₘ,app2 62.7 °C, 95 % CI [62.6, 62.9]; TteD: Tₘ,app1 43.7 °C, 95 % CI [43.6, 43.9], Tₘ,app2 53.2 °C, 95 % CI [53.1, 53.3]; TcuD: Tₘ,app1 46.0 °C, 95 % CI [45.8, 46.2], Tₘ,app2 53.1 °C, 95 % CI [53.0, 53.2]