Gayatri Prakash, Bo Chan Seo, Aaron Zeng, Frank X. Gu. “AERIS: an autonomous extensional rheology imaging-based system for rapid, high-throughput characterization of soft materials.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007811/v1

Values presented only in shear- or extensional-rheology plots or images were not transcribed. Platform-performance comparisons using Newtonian standards and glycerol were not retained

E6000 and E40000 Newtonian test fluids at 20 °C
Capillary-breakup extensional rheology Both fluids exhibited linear minimum-filament-diameter decay with minimal variation across replicates, where each replicate used a fresh loading and new top plate; breakup times were 2.34 ± 0.07 s for E6000 and 10.24 ± 0.44 s for E40000
Main, pp. 17–18
Glycerol
Capillary-breakup extensional rheology at 23.3 °C Viscosity 0.995 ± 0.060 Pa s
Capillary-breakup extensional rheology at 27.7 °C Viscosity 0.619 ± 0.023 Pa s
Main, p. 19
900 kDa HA solutions
Capillary-breakup extensional rheology Minimum filament diameter showed exponential thinning; higher HA concentrations gave longer breakup times and longer apparent extensional relaxation times
Main, p. 21
1 % w/v 900 kDa HA hydrogels with MgCl₂
Capillary-breakup extensional rheology, 7.5 mm plate separation Filament thinning was arrested at every MgCl₂ concentration across the 8.5 s observation window after a small initial decay; a non-crosslinked HA solution at the same polymer concentration showed exponential decay and broke within 2.5 s; arrest diameter increased with MgCl₂ concentration up to 60 mM and then showed no further change
Capillary-breakup extensional rheology, 10 mm plate separation The 20 mM MgCl₂ hydrogel produced an S-shaped curve and broke in 7.8 s; 30 and 50 mM formulations remained arrested over 30 s; 60 mM showed slight initial detachment followed by a plateau; 80 and 100 mM showed rapid complete detachment from the top plate
Main, pp. 22–23
1 % w/v HA with 50 mM MgCl₂ and 66.7 mM NaOH
Capillary-breakup extensional rheology, molecular-weight series For 50 kDa HA, MgCl₂ and NaOH, separately or together, left breakup time and apparent extensional relaxation time largely unchanged, with all four formulations breaking within 40 ms; for 100 kDa HA, MgCl₂ increased and NaOH decreased breakup and relaxation times; for 250 and 500 kDa HA, MgCl₂ or NaOH alone decreased both values relative to HA alone; adding MgCl₂ and NaOH together produced the highest breakup and relaxation times at each molecular weight, with 100 and 250 kDa filaments breaking and 500 kDa filament thinning arrested over 8.5 s
Cone-and-plate shear rheology Acceptable rheological profiles were not obtained for the low-molecular-weight HA formulations
Main, pp. 28–29; SI, p. 4
1500 kDa HA dilution series with MgCl₂, with and without NaOH
Capillary-breakup extensional rheology Formulations without NaOH broke in less than 1.2 s by exponential thinning, with apparent extensional relaxation time increasing with concentration; formulations containing NaOH showed arrested thinning over 8.5 s after an initial decay within the first second; higher concentrations showed slower initial decay and larger arrest diameters; 0.125 % w/v HA with 6 mM MgCl₂ broke within 3 ms without NaOH, while the corresponding formulation with NaOH showed arrested thinning