| AFM | Average length 381.8 nm; thickness, AFM height, 3.7 nm |
|---|---|
| Primary amine content | 1.7 mmol g⁻¹, corresponding to a degree of deacetylation of 32.2 % |
Ibrahim Abousharabia, Negin Vaziri, Arun Torris, Terhi J. Heino, Eero Kontturi, Vishnu Arumughan. “Anisotropic nanochitin hydrogels promoting guided cellular growth for bone defect repair.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007819/v1
Values presented only in AFM, SEM-EDX, WAXS, X-ray-tomography or mechanical-analysis plots or images were not transcribed. Simulated fluid-flow and cell-growth results were not transcribed. The main paper refers to supporting information, but no separate supporting information file was available on the ChemRxiv article page
| Water content | Above 97 % |
|---|---|
| EDX mapping | Crosslinkers were homogeneously distributed throughout the hydrogel matrices |
| SEM | Well-aligned pore channels were observed in transverse and longitudinal directions; freezing at −20 °C produced pore sizes of several hundred micrometers, while freezing at −196 °C produced pore sizes in the tens of micrometers; pore-wall orientation was predominantly axial at approximately 90°, with strong uniaxial ChNF alignment within the pore walls |
| WAXS | Azimuthal integration of the (110) diffraction rings showed distinct intensity peaks at 90° and 270° |
| WAXS-derived orientation factor | Orientation factors exceeded 0.5 and increased with ChNF content up to 1.2 wt. %; peak values were 0.8 for SPH-Ch1.2 % and 0.75 for STP-Ch1.2 %; orientation decreased above 1.2 wt. % |
| Uniaxial compression | Both hydrogels showed nonlinear J-shaped stress–strain responses in transverse and longitudinal directions; at 20 % strain, longitudinal-direction modulus was 59.48 % lower than the transverse direction for SPH and 3.49 % lower for STP; at 95 % strain, longitudinal-direction modulus exceeded the transverse direction by 11.13 % for SPH and 25.35 % for STP |
|---|---|
| X-ray tomography | Average pore diameters were approximately 142 µm for SPH-Ch1.2 % and 126 µm for STP-Ch1.2 % |