| UV absorbance | ~525 nm |
|---|---|
| Particle concentration, yeast B13G-AuNP | 2.91 × 10¹⁰ particles mL⁻¹ |
| Particle concentration, laminarin B13G-AuNP | 1.72 × 10¹² particles mL⁻¹ |
| Particle concentration, curdlan B13G-AuNP | 4.57 × 10¹⁰ particles mL⁻¹ |
| DLS, UV and TEM data | Image data were not transcribed |
John E. Chittum, Achyut Dahal, Joseph J. Barchi. “Synthesis and optimization of a nanoparticle vaccine to deliver tumor-associated glycopeptide immunogens against pancreatic cancer.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007945/v1
| FTIR, carbonyl stretch | 1700 wavenumbers |
|---|---|
| ¹H NMR | An additional methylene peak relative to starting B13G was reported; the source gives “at ppm” without a value |
| ¹³C NMR | A peak cluster around 178 ppm, consistent with carbonyl carbon signals |
| SEC-MALS, average molecular weight, CM-yeast B13G | 124.285 kDa |
| SEC-MALS, average molecular weight, CM-laminarin B13G | 8.476 kDa |
| SEC-MALS, average molecular weight, CM-curdlan B13G | 74.816 g mol⁻¹ |
| FTIR, ¹H NMR and ¹³C NMR spectra | Image data were not transcribed |
| ¹H NMR | Distinct peptide peaks were detected |
|---|
| y-AuNP | Z-average: 34.59 nm; average polydispersity: 0.257; AuNP core diameter range: 20–30 nm; average zeta potential: −35.7 mV |
|---|---|
| Laminarin-AuNP | Z-average: 29.33 nm; average polydispersity: 0.138; AuNP core diameter range: 20–30 nm; average zeta potential: −37.3 mV |
| CM-curdlan-AuNP | Z-average: 38.32 nm; average polydispersity: 0.216; AuNP core diameter range: 20–40 nm; average zeta potential: −46.9 mV |
| YMUC4-AuNP | Z-average: 25.25 nm; average polydispersity: 0.16; AuNP core diameter range: 15–25 nm; average zeta potential: −56.9 mV |
| LMUC4-AuNP | Z-average: 18.13 nm; average polydispersity: 0.047; AuNP core diameter range: 10–20 nm; average zeta potential: −37.3 mV |
| CMUC4-AuNP | Z-average: 37.55 nm; average polydispersity: 0.191; AuNP core diameter range: 20–30 nm; average zeta potential: −45.2 mV |
| YMUC4-5TF-AuNP | Z-average: 30.55 nm; average polydispersity: 0.27; AuNP core diameter range: 10–17 nm; average zeta potential: −42.4 mV |
| LMUC4-5TF-AuNP | Z-average: 23.53 nm; average polydispersity: 0.13; AuNP core diameter range: 10–15 nm; average zeta potential: −36.6 mV |
| UV, TEM, DLS and zeta-potential data | Image data were not transcribed |
| ¹H NMR (90:10 H₂O/D₂O) | A broad set of peaks between 7 and 9 ppm, assigned to amide proton resonances; signals for more upfield protons of hydrophobic side-chain resonances were also observed |
|---|---|
| ¹H NMR, MUC4 immunogen features | All NMR features of the immunogen were observed in the AuNP; the singlet marked “X” was assigned to a small amount of NHS remaining after purification |
| ¹H NMR spectra | Image data were not transcribed |