| Wide-field polarization-dependent fluorescence microscopy | No reproducible modulation of the stable fluorescence intensity was resolved along the fibers; the stable ensemble emission exhibited only a weak net polarization response at the diffraction-limited scale |
|---|---|
| SPD-STORM, image-wide Pearson correlation | R = −0.32 |
| SPD-STORM, neighboring blinking hotspots across 17 fibers | Mean distance 0.70 µm |
| SPD-STORM, longitudinal polarization-domain lengths | Median mixed-domain length approximately 0.75 µm; median X-preferential-domain length 1.58 µm; median Y-preferential-domain length 1.48 µm |
| SPD-STORM, representative longitudinal polarization domains | Approximately 0.5–4 µm was dominated by Polarization Y; approximately 9–11 µm was dominated by Polarization X; approximately 11–13 µm contained comparable contributions from both channels and was classified as a mixed domain |
| SPD-STORM, thick fibers with transverse FWHM greater than 300 nm | Polarization-dependent peaks occurred at different transverse locations, with their relative arrangement changing along the representative fiber. Additional thick fibers showed distinct Polarization X and Polarization Y distributions and variation between regions |
| Transmission electron microscopy | Thick fibers comprised multiple thinner fibrils associated laterally rather than a single monolithic structure |
| Scanning electron microscopy | A thick SachCHO fiber bundle was composed of laterally associated thinner fibrils; local changes in the trajectories of the constituent fibrils were consistent with intertwining within the bundle |
Peigen Yao, Alvaro Lopez-Acosta, Márton Gelléri, Mischa Bonn, Thomas M. Hermans, Xiaomin Liu. “Label-free mapping of nanoscale polarization heterogeneity in supramolecular fibers.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15007995/v1
Numerical values presented only in plotted profiles and distributions in the main paper, pp. 7–12, and SI, pp. 8–12, were not transcribed. Sequential-acquisition controls and correction factors were not retained
| Scanning electron microscopy | Fibers prepared at 4 °C were generally thinner and more curved; fibers prepared at 40 °C were thicker and exhibited more pronounced bundling of thin fibrils |
|---|---|
| Powder X-ray diffraction | The patterns at the three temperatures showed similar peak positions and overall profiles; the principal diffraction features were retained, although differences in their relative intensities were observed |
| SPD-STORM, mean nearest-neighbor hotspot distance | 4 °C: 0.91 µm; 20 °C: 0.70 µm; 40 °C: 0.64 µm |
| SPD-STORM, median X-preferential, Y-preferential and mixed-domain lengths; 14 fibers at 4 °C, 17 at 20 °C and 10 at 40 °C | 4 °C: 1.79, 1.66 and 0.64 µm; 20 °C: 1.58, 1.48 and 0.75 µm; 40 °C: 1.43, 1.28 and 1.27 µm |
| SPD-STORM, image-wide Pearson correlation | 4 °C: R = −0.16; 20 °C: R = −0.32; 40 °C: R = 0.60 |
| SPD-STORM, representative thick fiber assembled at 40 °C | The Polarization X and Polarization Y signals overlapped substantially within all three cross-sectional regions of interest, while their relative contributions varied along the fiber |