Tia Gibson, Thomas Floyd, Amaia Carrascal Miniño, Aishwarya Mishra, Juan Pellico, Kavitha Sunassee, Adam Jones, Truc T. Pham, Richard M. England, Marianne Ashford, Mariarosa Mazza, Timothy H. Witney, Rafael T. M. de Rosales. “Multimodal PET/fluorescence imaging for the preclinical evaluation of polysarcosine (pSar)-modified dendrimers: impact of pSar length on in vivo fate.” ChemRxiv (). https://doi.org/10.26434/chemrxiv.15008001/v1

The main paper refers to supporting information, but no separate supporting information file was available on the ChemRxiv article page

SD28, SD56 and SD100
¹H NMR Dendrimer: δ 1.0–1.9 ppm; pSar CH₃: δ 2.8–3.2 ppm; pSar CH₂: δ 4.0–4.6 ppm; end-group protons: δ 3.3–3.5 ppm; present at the expected relative proportions
Expected mass SD28: 74.0 kDa; SD56: 137.6 kDa; SD100: 237.5 kDa
¹H NMR, degree of polymerisation SD28: 30; SD56: 56; SD100: 122
MALDI-TOF-MS, degree of polymerisation and peak maximum molar mass SD28: DP 29, Mₚ 73.4 kDa; SD56: DP 54, Mₚ 131.3 kDa; SD100: DP 99, Mₚ 233.6 kDa
DLS, hydrodynamic diameter and size polydispersity index SD28: Dₕ 10.2 ± 0.1 nm, PDI 0.27 ± 0.01; SD56: Dₕ 14.1 ± 0.3 nm, PDI 0.35 ± 0.01; SD100: Dₕ 20.0 ± 0.4 nm, PDI 0.23 ± 0.01
MD-GPC/SEC, number-average molar mass, hydrodynamic diameter and molecular-weight dispersity SD28: Mₙ 72.0 kDa, Dₕ 11.64 nm, Đ 1.17; SD56: Mₙ 142.5 kDa, Dₕ 15.88 nm, Đ 1.01; SD100: Mₙ 262.2 kDa, Dₕ 20.36 nm, Đ 1.02
FPLC/SEC, retention volume SD28: 15.2 mL; SD56: 13.9 mL; SD100: 12.6 mL
Main, pp. 6–7
SD28, SD56 and SD100 before and after functionalisation and radiolabelling
DLS, unfunctionalised SD28: Dₕ 10.2 ± 0.1 nm, PDI 0.27 ± 0.01, zeta potential −0.62 ± 0.31 mV; SD56: Dₕ 14.1 ± 0.3 nm, PDI 0.35 ± 0.01, zeta potential −2.36 ± 0.45 mV; SD100: Dₕ 20.0 ± 0.4 nm, PDI 0.23 ± 0.01, zeta potential −1.44 ± 0.75 mV
DLS, DFO/OMe SD28: Dₕ 10.1 ± 2.3 nm, PDI 0.45 ± 0.02, zeta potential −3.18 ± 0.50 mV; SD56: Dₕ 15.9 ± 1.0 nm, PDI 0.22 ± 0.05, zeta potential −4.07 ± 0.49 mV; SD100: Dₕ 18.5 ± 0.5 nm, PDI 0.19 ± 0.03, zeta potential −0.20 ± 0.41 mV
SEC (method A), DFO/AF647/OMe, retention volume SD28: 15.0 mL; SD56: 13.4 mL; SD100: 12.3 mL
DLS, [⁸⁹Zr]Zr/DFO/OMe SD28: Dₕ 9.2 ± 0.1 nm, PDI 0.07 ± 0.04; SD56: Dₕ 14.1 ± 0.8 nm, PDI 0.18 ± 0.02; SD100: Dₕ 18.4 ± 0.8 nm, PDI 0.18 ± 0.01
SEC (method A), [⁸⁹Zr]Zr/DFO/AF647/OMe, retention volume SD28: 14.9 mL; SD56: 13.6 mL; SD100: 12.5 mL
DLS, [⁸⁹Zr]Zr/DFO/AF647/OMe, zeta potential SD28: −5.39 ± 2.00 mV; SD56: −2.50 ± 0.98 mV; SD100: −3.37 ± 0.40 mV
DLS and SEC plots Image data were not transcribed
Main, pp. 7–8, 10
[⁸⁹Zr]Zr/DFO/AF647/OMe-modified SD28, SD56 and SD100
SEC, radiochemical purity (post-purification; mean ± SD, n = 3) SD28: 96 ± 3 %; SD56: 95 ± 3 %; SD100: 95 ± 2 %
Radiochemical purity and stability plots Image data were not transcribed