Model name: threestage
<br>
17-Nov-2021 22:54:45
<br>
<br>
Model parameters
<table border='1px'>
<tr>
<td>
Parameter
</td>
<td>
BoundaryCondition
</td>
<td>
ConstantValue
</td>
<td>
Value
</td>
<td>
Units
</td>
<td>
Notes
</td>
</tr>
<tr>
<td>
k_ImpB_on
</td>
<td>
false
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
k_ImpB_off
</td>
<td>
false
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
</table>
<br>
<br>
Compartments
<table border='1px'>
<tr>
<td>
Name
</td>
<td>
ConstantCapacity
</td>
<td>
Capacity
</td>
<td>
CapacityUnits
</td>
<td>
Notes
</td>
</tr>
<tr>
<td>
C
</td>
<td>
true
</td>
<td>
     1

</td>
<td>
picoliter
</td>
<td>
</td>
</tr>
</table>
<br>
<br>
Species
<table border='1px'>
<tr>
<td>
Compartment
</td>
<td>
Name
</td>
<td>
ConstantAmount
</td>
<td>
InitialAmount
</td>
<td>
InitialAmountUnits
</td>
<td>
Notes
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NPC(c)
</td>
<td>
false
</td>
<td>
    0.0100

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpB(c)
</td>
<td>
false
</td>
<td>
     1

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpB·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpB(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA(c)
</td>
<td>
false
</td>
<td>
     1

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·ImpB·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·ImpB(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS(c)
</td>
<td>
false
</td>
<td>
     1

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS·ImpA·ImpB(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS·ImpA·ImpB·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
Ran·GTP(n)
</td>
<td>
false
</td>
<td>
     3

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
Ran·GTP·ImpB·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS(c)
</td>
<td>
false
</td>
<td>
     1

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
RanGAP
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
RanBP1
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
Ran·GDP(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS·Ran·GTP(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·CAS·Ran·GTP(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·CAS·Ran·GTP·NPC(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·ImpB(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·CAS·Ran·GTP(c)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS·ImpA·ImpB(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·ImpB·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpB·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NPC(n)
</td>
<td>
false
</td>
<td>
    0.0100

</td>
<td>
uM
</td>
<td>
100 Kaps per NPC

</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS·ImpA·ImpB·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
Ran·GTP·ImpB·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·CAS·Ran·GTP·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS·NPC(n)
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·ImpB·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpB·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NPC
</td>
<td>
false
</td>
<td>
     1

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
NLS·ImpA·ImpB·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
Ran·GTP·ImpB·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
ImpA·CAS·Ran·GTP·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
<tr>
<td>
C
</td>
<td>
CAS·NPC
</td>
<td>
false
</td>
<td>
     0

</td>
<td>
uM
</td>
<td>
</td>
</tr>
</table>
<br>
<br>
Reaction parameters
<table border='1px'>
<tr>
<td>
ReactionName
</td>
<td>
Reaction
</td>
<td>
Parameter
</td>
<td>
ConstantValue
</td>
<td>
Value
</td>
<td>
Units
</td>
<td>
Notes
</td>
</tr>
<tr>
<td>
r7
</td>
<td>
[ImpA(c)] + [ImpB(c)] <-> [ImpA·ImpB(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.4900

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r7
</td>
<td>
[ImpA(c)] + [ImpB(c)] <-> [ImpA·ImpB(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
    0.0025

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r9
</td>
<td>
[ImpA(c)] + [ImpB·NPC(c)] -> [ImpA·ImpB·NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.4900

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r9
</td>
<td>
[ImpA(c)] + [ImpB·NPC(c)] -> [ImpA·ImpB·NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
    0.0025

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r10
</td>
<td>
[NLS(c)] + [ImpA·ImpB(c)] <-> [NLS·ImpA·ImpB(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1500

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r10
</td>
<td>
[NLS(c)] + [ImpA·ImpB(c)] <-> [NLS·ImpA·ImpB(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   4.4000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r11
</td>
<td>
[NLS·ImpA·ImpB(c)] + [NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r11
</td>
<td>
[NLS·ImpA·ImpB(c)] + [NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r12
</td>
<td>
[NLS(c)] + [ImpA·ImpB·NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1500

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r12
</td>
<td>
[NLS(c)] + [ImpA·ImpB·NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   4.4000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
knockoff
</td>
<td>
[Ran·GTP(n)] + [NLS·ImpA·ImpB·NPC(n)] -> [Ran·GTP·ImpB·NPC(n)] + [ImpA(n)] + [NLS(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.0200

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
hydrolysis of Ran·GTP·ImpB·NPC
</td>
<td>
[Ran·GTP·ImpB·NPC(c)] + RanGAP + RanBP1 -> [Ran·GDP(c)] + [ImpB·NPC(c)] + RanGAP + RanBP1
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1000

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with NPC(c)
</td>
<td>
[CAS(c)] + [NPC(c)] <-> [CAS·NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with NPC(c)
</td>
<td>
[CAS(c)] + [NPC(c)] <-> [CAS·NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with NPC(n)
</td>
<td>
[CAS(n)] + [NPC(n)] <-> [CAS·NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with NPC(n)
</td>
<td>
[CAS(n)] + [NPC(n)] <-> [CAS·NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with Ran
</td>
<td>
[Ran·GTP(n)] + [CAS(n)] <-> [CAS·Ran·GTP(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.0100

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS with Ran
</td>
<td>
[Ran·GTP(n)] + [CAS(n)] <-> [CAS·Ran·GTP(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
    0.0150

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Complex CAS with NPC(n)
</td>
<td>
[ImpA·CAS·Ran·GTP(n)] + [NPC(n)] <-> [ImpA·CAS·Ran·GTP·NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Complex CAS with NPC(n)
</td>
<td>
[ImpA·CAS·Ran·GTP(n)] + [NPC(n)] <-> [ImpA·CAS·Ran·GTP·NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
hydrolysis of ImpA·CAS·Ran·GTP·NPC
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(c)] + RanGAP + RanBP1 -> [Ran·GDP(c)] + [ImpA(c)] + [CAS·NPC(c)] + RanGAP + RanBP1
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1000

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS·Ran with ImpA
</td>
<td>
[ImpA(n)] + [CAS·Ran·GTP(n)] <-> [ImpA·CAS·Ran·GTP(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1000

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
CAS·Ran with ImpA
</td>
<td>
[ImpA(n)] + [CAS·Ran·GTP(n)] <-> [ImpA·CAS·Ran·GTP(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Ran gradient
</td>
<td>
[Ran·GDP(c)] -> [Ran·GTP(n)]
</td>
<td>
k_pump
</td>
<td>
true
</td>
<td>
    0.0100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Complex CAS with NPC(c)
</td>
<td>
[ImpA·CAS·Ran·GTP(c)] + [NPC(c)] <-> [ImpA·CAS·Ran·GTP·NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Complex CAS with NPC(c)
</td>
<td>
[ImpA·CAS·Ran·GTP(c)] + [NPC(c)] <-> [ImpA·CAS·Ran·GTP·NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Replenish NLS
</td>
<td>
null -> [NLS(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
     0

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r12_1
</td>
<td>
[NLS(n)] + [ImpA·ImpB·NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1500

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r12_1
</td>
<td>
[NLS(n)] + [ImpA·ImpB·NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   4.4000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r10_1
</td>
<td>
[NLS(n)] + [ImpA·ImpB(n)] <-> [NLS·ImpA·ImpB(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
    0.1500

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r10_1
</td>
<td>
[NLS(n)] + [ImpA·ImpB(n)] <-> [NLS·ImpA·ImpB(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   4.4000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r11_1
</td>
<td>
[NLS·ImpA·ImpB(n)] + [NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   1.0000e-03

</td>
<td>
1/uM/s
</td>
<td>
</td>
</tr>
<tr>
<td>
r11_1
</td>
<td>
[NLS·ImpA·ImpB(n)] + [NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   1.0000e-04

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Passive diffusion
</td>
<td>
[NLS(n)] <-> [NLS(c)]
</td>
<td>
k_diff
</td>
<td>
true
</td>
<td>
     0

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·ImpB·NPC from (c)
</td>
<td>
[ImpA·ImpB·NPC(c)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·ImpB·NPC from (c)
</td>
<td>
[ImpA·ImpB·NPC(c)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
ImpA·ImpB·NPC from (n)
</td>
<td>
[ImpA·ImpB·NPC(n)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
ImpA·ImpB·NPC from (n)
</td>
<td>
[ImpA·ImpB·NPC(n)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (c)
</td>
<td>
[ImpB·NPC(c)] + NPC <-> [ImpB·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (c)
</td>
<td>
[ImpB·NPC(c)] + NPC <-> [ImpB·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (n)
</td>
<td>
[ImpB·NPC(n)] + NPC <-> [ImpB·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (n)
</td>
<td>
[ImpB·NPC(n)] + NPC <-> [ImpB·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (c)
</td>
<td>
[NLS·ImpA·ImpB·NPC(c)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (c)
</td>
<td>
[NLS·ImpA·ImpB·NPC(c)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (n)
</td>
<td>
[NLS·ImpA·ImpB·NPC(n)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (n)
</td>
<td>
[NLS·ImpA·ImpB·NPC(n)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (c)
</td>
<td>
[Ran·GTP·ImpB·NPC(c)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (c)
</td>
<td>
[Ran·GTP·ImpB·NPC(c)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (n)
</td>
<td>
[Ran·GTP·ImpB·NPC(n)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (n)
</td>
<td>
[Ran·GTP·ImpB·NPC(n)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (c)
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(c)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (c)
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(c)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (n)
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(n)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (n)
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(n)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (c)
</td>
<td>
[CAS·NPC(c)] + NPC <-> [CAS·NPC] + [NPC(c)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (c)
</td>
<td>
[CAS·NPC(c)] + NPC <-> [CAS·NPC] + [NPC(c)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (n)
</td>
<td>
[CAS·NPC(n)] + NPC <-> [CAS·NPC] + [NPC(n)]
</td>
<td>
kf
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (n)
</td>
<td>
[CAS·NPC(n)] + NPC <-> [CAS·NPC] + [NPC(n)]
</td>
<td>
kr
</td>
<td>
true
</td>
<td>
   100

</td>
<td>
1/s
</td>
<td>
</td>
</tr>
</table>
<br>
<br>
Reactions
<table border='1px'>
<tr>
<td>
Name
</td>
<td>
Active
</td>
<td>
Reversible
</td>
<td>
Reaction
</td>
<td>
ReactionRate
</td>
<td>
Notes
</td>
</tr>
<tr>
<td>
r1
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpB(c)] + [NPC(c)] <-> [ImpB·NPC(c)]
</td>
<td>
k_ImpB_on*C.[ImpB(c)]*C.[NPC(c)]-k_ImpB_off*C.[ImpB·NPC(c)]
</td>
<td>
Constants: LK, email of Apr 2
</td>
</tr>
<tr>
<td>
r2
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpB(n)] + [NPC(n)] <-> [ImpB·NPC(n)]
</td>
<td>
k_ImpB_on*C.[ImpB(n)]*C.[NPC(n)]-k_ImpB_off*C.[ImpB·NPC(n)]
</td>
<td>
Constants: LK, email of Apr 2
</td>
</tr>
<tr>
<td>
r8
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·ImpB(c)] + [NPC(c)] <-> [ImpA·ImpB·NPC(c)]
</td>
<td>
k_ImpB_on*[ImpA·ImpB(c)]*[NPC(c)]-k_ImpB_off*[ImpA·ImpB·NPC(c)]
</td>
<td>
Same as ImpB+NPC

</td>
</tr>
<tr>
<td>
r7
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA(c)] + [ImpB(c)] <-> [ImpA·ImpB(c)]
</td>
<td>
r7.kf*[ImpA(c)]*[ImpB(c)]-r7.kr*[ImpA·ImpB(c)]
</td>
<td>
Catimel et al, 2001, Table I
</td>
</tr>
<tr>
<td>
r9
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[ImpA(c)] + [ImpB·NPC(c)] -> [ImpA·ImpB·NPC(c)]
</td>
<td>
r9.kf*[ImpA(c)]*[ImpB·NPC(c)]-r9.kr*[ImpA·ImpB·NPC(c)]
</td>
<td>
Same as ImpA+ImpB

</td>
</tr>
<tr>
<td>
r10
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS(c)] + [ImpA·ImpB(c)] <-> [NLS·ImpA·ImpB(c)]
</td>
<td>
kf*[NLS(c)]*[ImpA·ImpB(c)]-kr*[NLS·ImpA·ImpB(c)]
</td>
<td>
Catimel et al, 2001, Table I
</td>
</tr>
<tr>
<td>
r11
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS·ImpA·ImpB(c)] + [NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kf*[NLS·ImpA·ImpB(c)]*[NPC(c)]-kr*[NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
Same as ImpB+NPC

</td>
</tr>
<tr>
<td>
r12
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS(c)] + [ImpA·ImpB·NPC(c)] <-> [NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
kf*[NLS(c)]*[ImpA·ImpB·NPC(c)]-kr*[NLS·ImpA·ImpB·NPC(c)]
</td>
<td>
Same as r10

</td>
</tr>
<tr>
<td>
knockoff
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[Ran·GTP(n)] + [NLS·ImpA·ImpB·NPC(n)] -> [Ran·GTP·ImpB·NPC(n)] + [ImpA(n)] + [NLS(n)]
</td>
<td>
knockoff.kf*[Ran·GTP(n)]*[NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
"knockoff"
</td>
</tr>
<tr>
<td>
hydrolysis of Ran·GTP·ImpB·NPC
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[Ran·GTP·ImpB·NPC(c)] + RanGAP + RanBP1 -> [Ran·GDP(c)] + [ImpB·NPC(c)] + RanGAP + RanBP1
</td>
<td>
kf*[Ran·GTP·ImpB·NPC(c)]
</td>
<td>
guesstimate
</td>
</tr>
<tr>
<td>
CAS with NPC(c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[CAS(c)] + [NPC(c)] <-> [CAS·NPC(c)]
</td>
<td>
kf*[CAS(c)]*[NPC(c)]-kr*[CAS·NPC(c)]
</td>
<td>
guesstimate

</td>
</tr>
<tr>
<td>
CAS with NPC(n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[CAS(n)] + [NPC(n)] <-> [CAS·NPC(n)]
</td>
<td>
kf*[CAS(n)]*[NPC(n)]-kr*[CAS·NPC(n)]
</td>
<td>
(copy)
</td>
</tr>
<tr>
<td>
CAS with Ran
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[Ran·GTP(n)] + [CAS(n)] <-> [CAS·Ran·GTP(n)]
</td>
<td>
kf*[Ran·GTP(n)]*[CAS(n)]-kr*[CAS·Ran·GTP(n)]
</td>
<td>
"Estimate" from RM 2005, Table II

kD = 1.44 uM (LK)

</td>
</tr>
<tr>
<td>
Complex CAS with NPC(n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·CAS·Ran·GTP(n)] + [NPC(n)] <-> [ImpA·CAS·Ran·GTP·NPC(n)]
</td>
<td>
kf*[ImpA·CAS·Ran·GTP(n)]*[NPC(n)]-kr*[ImpA·CAS·Ran·GTP·NPC(n)]
</td>
<td>
(copy)
</td>
</tr>
<tr>
<td>
hydrolysis of ImpA·CAS·Ran·GTP·NPC
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(c)] + RanGAP + RanBP1 -> [Ran·GDP(c)] + [ImpA(c)] + [CAS·NPC(c)] + RanGAP + RanBP1
</td>
<td>
kf*[ImpA·CAS·Ran·GTP·NPC(c)]
</td>
<td>
guesstimate
</td>
</tr>
<tr>
<td>
CAS·Ran with ImpA
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA(n)] + [CAS·Ran·GTP(n)] <-> [ImpA·CAS·Ran·GTP(n)]
</td>
<td>
kf*[ImpA(n)]*[CAS·Ran·GTP(n)] - kr * [ImpA·CAS·Ran·GTP(n)]
</td>
<td>
Est. based on RM 2005, Table II
</td>
</tr>
<tr>
<td>
Ran gradient
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[Ran·GDP(c)] -> [Ran·GTP(n)]
</td>
<td>
k_pump*[Ran·GDP(c)]
</td>
<td>
guesstimate
</td>
</tr>
<tr>
<td>
r8_1
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·ImpB(n)] + [NPC(n)] <-> [ImpA·ImpB·NPC(n)]
</td>
<td>
k_ImpB_on*C.[ImpA·ImpB(n)]*C.[NPC(n)]-k_ImpB_off*C.[ImpA·ImpB·NPC(n)]
</td>
<td>
Same as ImpB+NPC

</td>
</tr>
<tr>
<td>
r9_1
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[ImpA(n)] + [ImpB·NPC(n)] -> [ImpA·ImpB·NPC(n)]
</td>
<td>
r9.kf*C.[ImpA(n)]*C.[ImpB·NPC(n)]-r9.kr*C.[ImpA·ImpB·NPC(n)]
</td>
<td>
Same as ImpA+ImpB

</td>
</tr>
<tr>
<td>
r7_1
</td>
<td>
false
</td>
<td>
true
</td>
<td>
[ImpA(n)] + [ImpB(n)] <-> [ImpA·ImpB(n)]
</td>
<td>
r7.kf*C.[ImpA(n)]*C.[ImpB(n)]-r7.kr*C.[ImpA·ImpB(n)]
</td>
<td>
see r7

Off, because no corresponding "knockoff" reaction.
</td>
</tr>
<tr>
<td>
Complex CAS with NPC(c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·CAS·Ran·GTP(c)] + [NPC(c)] <-> [ImpA·CAS·Ran·GTP·NPC(c)]
</td>
<td>
kf*C.[ImpA·CAS·Ran·GTP(c)]*C.[NPC(c)]-kr*C.[ImpA·CAS·Ran·GTP·NPC(c)]
</td>
<td>
guesstimate
10^3 -- 10^4  1/M/s

koff:
1e-4 (wt)
1e-2 (mutant)

</td>
</tr>
<tr>
<td>
hydrolysis of ImpA·CAS·Ran·GTP(c)
</td>
<td>
true
</td>
<td>
false
</td>
<td>
[ImpA·CAS·Ran·GTP(c)] + RanGAP + RanBP1 -> [Ran·GDP(c)] + [ImpA(c)] + [CAS(c)] + RanGAP + RanBP1
</td>
<td>
[hydrolysis of ImpA·CAS·Ran·GTP·NPC].kf*C.[ImpA·CAS·Ran·GTP(c)]
</td>
<td>
guesstimate
</td>
</tr>
<tr>
<td>
Replenish NLS
</td>
<td>
true
</td>
<td>
false
</td>
<td>
null -> [NLS(c)]
</td>
<td>
kf*[NLS(n)]
</td>
<td>
</td>
</tr>
<tr>
<td>
r12_1
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS(n)] + [ImpA·ImpB·NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kf*C.[NLS(n)]*C.[ImpA·ImpB·NPC(n)]-kr*C.[NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
See r12
</td>
</tr>
<tr>
<td>
r10_1
</td>
<td>
false
</td>
<td>
true
</td>
<td>
[NLS(n)] + [ImpA·ImpB(n)] <-> [NLS·ImpA·ImpB(n)]
</td>
<td>
kf*C.[NLS(n)]*C.[ImpA·ImpB(n)]-kr*C.[NLS·ImpA·ImpB(n)]
</td>
<td>
See r10

Off, because no corresponding "knockoff" reaction.
</td>
</tr>
<tr>
<td>
r11_1
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS·ImpA·ImpB(n)] + [NPC(n)] <-> [NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
kf*C.[NLS·ImpA·ImpB(n)]*C.[NPC(n)]-kr*C.[NLS·ImpA·ImpB·NPC(n)]
</td>
<td>
See r11
</td>
</tr>
<tr>
<td>
Passive diffusion
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS(n)] <-> [NLS(c)]
</td>
<td>
k_diff * ([NLS(n)] - [NLS(c)])
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·ImpB·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·ImpB·NPC(c)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf*[ImpA·ImpB·NPC(c)] - kr*[ImpA·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
ImpA·ImpB·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·ImpB·NPC(n)] + NPC <-> [ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf*[ImpA·ImpB·NPC(n)] - kr*[ImpA·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpB·NPC(c)] + NPC <-> [ImpB·NPC] + [NPC(c)]
</td>
<td>
kf*[ImpB·NPC(c)] - kr*[ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpB·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpB·NPC(n)] + NPC <-> [ImpB·NPC] + [NPC(n)]
</td>
<td>
kf*[ImpB·NPC(n)] - kr*[ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS·ImpA·ImpB·NPC(c)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf*[NLS·ImpA·ImpB·NPC(c)] - kr*[NLS·ImpA·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry NLS·ImpA·ImpB·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[NLS·ImpA·ImpB·NPC(n)] + NPC <-> [NLS·ImpA·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf*[NLS·ImpA·ImpB·NPC(n)] - kr*[NLS·ImpA·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[Ran·GTP·ImpB·NPC(c)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(c)]
</td>
<td>
kf*[Ran·GTP·ImpB·NPC(c)] - kr*[Ran·GTP·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry Ran·GTP·ImpB·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[Ran·GTP·ImpB·NPC(n)] + NPC <-> [Ran·GTP·ImpB·NPC] + [NPC(n)]
</td>
<td>
kf*[Ran·GTP·ImpB·NPC(n)] - kr*[Ran·GTP·ImpB·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(c)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(c)]
</td>
<td>
kf*[ImpA·CAS·Ran·GTP·NPC(c)] - kr*[ImpA·CAS·Ran·GTP·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry ImpA·CAS·Ran·GTP·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[ImpA·CAS·Ran·GTP·NPC(n)] + NPC <-> [ImpA·CAS·Ran·GTP·NPC] + [NPC(n)]
</td>
<td>
kf*[ImpA·CAS·Ran·GTP·NPC(n)] - kr*[ImpA·CAS·Ran·GTP·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (c)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[CAS·NPC(c)] + NPC <-> [CAS·NPC] + [NPC(c)]
</td>
<td>
kf*[CAS·NPC(c)] - kr*[CAS·NPC]
</td>
<td>
</td>
</tr>
<tr>
<td>
Entry CAS·NPC from (n)
</td>
<td>
true
</td>
<td>
true
</td>
<td>
[CAS·NPC(n)] + NPC <-> [CAS·NPC] + [NPC(n)]
</td>
<td>
kf*[CAS·NPC(n)] - kr*[CAS·NPC]
</td>
<td>
</td>
</tr>
</table>
<br>
<br>
Equations dump
<pre>
ODEs:
d([NPC(c)])/dt = 1/C*(-r1 - r8 - r11 - [CAS with NPC(c)] - [Complex CAS with NPC(c)] + [Entry ImpA·ImpB·NPC from (c)] + [Entry ImpB·NPC from (c)] + [Entry NLS·ImpA·ImpB·NPC from (c)] + [Entry Ran·GTP·ImpB·NPC from (c)] + [Entry ImpA·CAS·Ran·GTP·NPC from (c)] + [Entry CAS·NPC from (c)])
d([ImpB(c)])/dt = 1/C*(-r1 - r7)
d([ImpB·NPC(c)])/dt = 1/C*(r1 - r9 + [hydrolysis of Ran·GTP·ImpB·NPC] - [Entry ImpB·NPC from (c)])
d([ImpB(n)])/dt = 1/C*(-r2)
d([ImpA(c)])/dt = 1/C*(-r7 - r9 + [hydrolysis of ImpA·CAS·Ran·GTP·NPC] + [hydrolysis of ImpA·CAS·Ran·GTP(c)])
d([ImpA·ImpB·NPC(c)])/dt = 1/C*(r8 + r9 - r12 - [Entry ImpA·ImpB·NPC from (c)])
d([ImpA·ImpB(c)])/dt = 1/C*(-r8 + r7 - r10)
d([NLS(c)])/dt = 1/C*(-r10 - r12 + [Replenish NLS] + [Passive diffusion])
d([NLS·ImpA·ImpB(c)])/dt = 1/C*(r10 - r11)
d([NLS·ImpA·ImpB·NPC(c)])/dt = 1/C*(r11 + r12 - [Entry NLS·ImpA·ImpB·NPC from (c)])
d([Ran·GTP(n)])/dt = 1/C*(-knockoff - [CAS with Ran] + [Ran gradient])
d([NLS(n)])/dt = 1/C*(knockoff - r12_1 - [Passive diffusion])
d([Ran·GTP·ImpB·NPC(c)])/dt = 1/C*(-[hydrolysis of Ran·GTP·ImpB·NPC] - [Entry Ran·GTP·ImpB·NPC from (c)])
d([CAS(c)])/dt = 1/C*(-[CAS with NPC(c)] + [hydrolysis of ImpA·CAS·Ran·GTP(c)])
d([Ran·GDP(c)])/dt = 1/C*([hydrolysis of Ran·GTP·ImpB·NPC] + [hydrolysis of ImpA·CAS·Ran·GTP·NPC] - [Ran gradient] + [hydrolysis of ImpA·CAS·Ran·GTP(c)])
d([CAS·NPC(c)])/dt = 1/C*([CAS with NPC(c)] + [hydrolysis of ImpA·CAS·Ran·GTP·NPC] - [Entry CAS·NPC from (c)])
d([CAS(n)])/dt = 1/C*(-[CAS with NPC(n)] - [CAS with Ran])
d([CAS·Ran·GTP(n)])/dt = 1/C*([CAS with Ran] - [CAS·Ran with ImpA])
d([ImpA·CAS·Ran·GTP(n)])/dt = 1/C*(-[Complex CAS with NPC(n)] + [CAS·Ran with ImpA])
d([ImpA·CAS·Ran·GTP·NPC(c)])/dt = 1/C*(-[hydrolysis of ImpA·CAS·Ran·GTP·NPC] + [Complex CAS with NPC(c)] - [Entry ImpA·CAS·Ran·GTP·NPC from (c)])
d([ImpA(n)])/dt = 1/C*(knockoff - [CAS·Ran with ImpA] - r9_1)
d([ImpA·ImpB(n)])/dt = 1/C*(-r8_1)
d([ImpA·CAS·Ran·GTP(c)])/dt = 1/C*(-[Complex CAS with NPC(c)] - [hydrolysis of ImpA·CAS·Ran·GTP(c)])
d([NLS·ImpA·ImpB(n)])/dt = 1/C*(-r11_1)
d([ImpA·ImpB·NPC(n)])/dt = 1/C*(r8_1 + r9_1 - r12_1 - [ImpA·ImpB·NPC from (n)])
d([ImpB·NPC(n)])/dt = 1/C*(r2 - r9_1 - [Entry ImpB·NPC from (n)])
d([NPC(n)])/dt = 1/C*(-r2 - [CAS with NPC(n)] - [Complex CAS with NPC(n)] - r8_1 - r11_1 + [ImpA·ImpB·NPC from (n)] + [Entry ImpB·NPC from (n)] + [Entry NLS·ImpA·ImpB·NPC from (n)] + [Entry Ran·GTP·ImpB·NPC from (n)] + [Entry ImpA·CAS·Ran·GTP·NPC from (n)] + [Entry CAS·NPC from (n)])
d([NLS·ImpA·ImpB·NPC(n)])/dt = 1/C*(-knockoff + r12_1 + r11_1 - [Entry NLS·ImpA·ImpB·NPC from (n)])
d([Ran·GTP·ImpB·NPC(n)])/dt = 1/C*(knockoff - [Entry Ran·GTP·ImpB·NPC from (n)])
d([ImpA·CAS·Ran·GTP·NPC(n)])/dt = 1/C*([Complex CAS with NPC(n)] - [Entry ImpA·CAS·Ran·GTP·NPC from (n)])
d([CAS·NPC(n)])/dt = 1/C*([CAS with NPC(n)] - [Entry CAS·NPC from (n)])
d([ImpA·ImpB·NPC])/dt = 1/C*([Entry ImpA·ImpB·NPC from (c)] + [ImpA·ImpB·NPC from (n)])
d([ImpB·NPC])/dt = 1/C*([Entry ImpB·NPC from (c)] + [Entry ImpB·NPC from (n)])
d(NPC)/dt = 1/C*(-[Entry ImpA·ImpB·NPC from (c)] - [ImpA·ImpB·NPC from (n)] - [Entry ImpB·NPC from (c)] - [Entry ImpB·NPC from (n)] - [Entry NLS·ImpA·ImpB·NPC from (c)] - [Entry NLS·ImpA·ImpB·NPC from (n)] - [Entry Ran·GTP·ImpB·NPC from (c)] - [Entry Ran·GTP·ImpB·NPC from (n)] - [Entry ImpA·CAS·Ran·GTP·NPC from (c)] - [Entry ImpA·CAS·Ran·GTP·NPC from (n)] - [Entry CAS·NPC from (c)] - [Entry CAS·NPC from (n)])
d([NLS·ImpA·ImpB·NPC])/dt = 1/C*([Entry NLS·ImpA·ImpB·NPC from (c)] + [Entry NLS·ImpA·ImpB·NPC from (n)])
d([Ran·GTP·ImpB·NPC])/dt = 1/C*([Entry Ran·GTP·ImpB·NPC from (c)] + [Entry Ran·GTP·ImpB·NPC from (n)])
d([ImpA·CAS·Ran·GTP·NPC])/dt = 1/C*([Entry ImpA·CAS·Ran·GTP·NPC from (c)] + [Entry ImpA·CAS·Ran·GTP·NPC from (n)])
d([CAS·NPC])/dt = 1/C*([Entry CAS·NPC from (c)] + [Entry CAS·NPC from (n)])

Fluxes:
r1 = (k_ImpB_on*[ImpB(c)]*[NPC(c)]-k_ImpB_off*[ImpB·NPC(c)])*C
r2 = (k_ImpB_on*[ImpB(n)]*[NPC(n)]-k_ImpB_off*[ImpB·NPC(n)])*C
r8 = (k_ImpB_on*[ImpA·ImpB(c)]*[NPC(c)]-k_ImpB_off*[ImpA·ImpB·NPC(c)])*C
r7 = (r7.kf*[ImpA(c)]*[ImpB(c)]-r7.kr*[ImpA·ImpB(c)])*C
r9 = (r9.kf*[ImpA(c)]*[ImpB·NPC(c)]-r9.kr*[ImpA·ImpB·NPC(c)])*C
r10 = (r10.kf*[NLS(c)]*[ImpA·ImpB(c)]-r10.kr*[NLS·ImpA·ImpB(c)])*C
r11 = (r11.kf*[NLS·ImpA·ImpB(c)]*[NPC(c)]-r11.kr*[NLS·ImpA·ImpB·NPC(c)])*C
r12 = (r12.kf*[NLS(c)]*[ImpA·ImpB·NPC(c)]-r12.kr*[NLS·ImpA·ImpB·NPC(c)])*C
knockoff = (knockoff.kf*[Ran·GTP(n)]*[NLS·ImpA·ImpB·NPC(n)])*C
[hydrolysis of Ran·GTP·ImpB·NPC] = ([hydrolysis of Ran·GTP·ImpB·NPC].kf*[Ran·GTP·ImpB·NPC(c)])*C
[CAS with NPC(c)] = ([CAS with NPC(c)].kf*[CAS(c)]*[NPC(c)]-[CAS with NPC(c)].kr*[CAS·NPC(c)])*C
[CAS with NPC(n)] = ([CAS with NPC(n)].kf*[CAS(n)]*[NPC(n)]-[CAS with NPC(n)].kr*[CAS·NPC(n)])*C
[CAS with Ran] = ([CAS with Ran].kf*[Ran·GTP(n)]*[CAS(n)]-[CAS with Ran].kr*[CAS·Ran·GTP(n)])*C
[Complex CAS with NPC(n)] = ([Complex CAS with NPC(n)].kf*[ImpA·CAS·Ran·GTP(n)]*[NPC(n)]-[Complex CAS with NPC(n)].kr*[ImpA·CAS·Ran·GTP·NPC(n)])*C
[hydrolysis of ImpA·CAS·Ran·GTP·NPC] = ([hydrolysis of ImpA·CAS·Ran·GTP·NPC].kf*[ImpA·CAS·Ran·GTP·NPC(c)])*C
[CAS·Ran with ImpA] = ([CAS·Ran with ImpA].kf*[ImpA(n)]*[CAS·Ran·GTP(n)]-[CAS·Ran with ImpA].kr*[ImpA·CAS·Ran·GTP(n)])*C
[Ran gradient] = (k_pump*[Ran·GDP(c)])*C
r8_1 = (k_ImpB_on*[ImpA·ImpB(n)]*[NPC(n)]-k_ImpB_off*[ImpA·ImpB·NPC(n)])*C
r9_1 = (r9.kf*[ImpA(n)]*[ImpB·NPC(n)]-r9.kr*[ImpA·ImpB·NPC(n)])*C
[Complex CAS with NPC(c)] = ([Complex CAS with NPC(c)].kf*[ImpA·CAS·Ran·GTP(c)]*[NPC(c)]-[Complex CAS with NPC(c)].kr*[ImpA·CAS·Ran·GTP·NPC(c)])*C
[hydrolysis of ImpA·CAS·Ran·GTP(c)] = ([hydrolysis of ImpA·CAS·Ran·GTP·NPC].kf*[ImpA·CAS·Ran·GTP(c)])*C
[Replenish NLS] = ([Replenish NLS].kf*[NLS(n)])*C
r12_1 = (r12_1.kf*[NLS(n)]*[ImpA·ImpB·NPC(n)]-r12_1.kr*[NLS·ImpA·ImpB·NPC(n)])*C
r11_1 = (r11_1.kf*[NLS·ImpA·ImpB(n)]*[NPC(n)]-r11_1.kr*[NLS·ImpA·ImpB·NPC(n)])*C
[Passive diffusion] = (k_diff*([NLS(n)]-[NLS(c)]))*C
[Entry ImpA·ImpB·NPC from (c)] = ([Entry ImpA·ImpB·NPC from (c)].kf*[ImpA·ImpB·NPC(c)]-[Entry ImpA·ImpB·NPC from (c)].kr*[ImpA·ImpB·NPC])*C
[ImpA·ImpB·NPC from (n)] = ([ImpA·ImpB·NPC from (n)].kf*[ImpA·ImpB·NPC(n)]-[ImpA·ImpB·NPC from (n)].kr*[ImpA·ImpB·NPC])*C
[Entry ImpB·NPC from (c)] = ([Entry ImpB·NPC from (c)].kf*[ImpB·NPC(c)]-[Entry ImpB·NPC from (c)].kr*[ImpB·NPC])*C
[Entry ImpB·NPC from (n)] = ([Entry ImpB·NPC from (n)].kf*[ImpB·NPC(n)]-[Entry ImpB·NPC from (n)].kr*[ImpB·NPC])*C
[Entry NLS·ImpA·ImpB·NPC from (c)] = ([Entry NLS·ImpA·ImpB·NPC from (c)].kf*[NLS·ImpA·ImpB·NPC(c)]-[Entry NLS·ImpA·ImpB·NPC from (c)].kr*[NLS·ImpA·ImpB·NPC])*C
[Entry NLS·ImpA·ImpB·NPC from (n)] = ([Entry NLS·ImpA·ImpB·NPC from (n)].kf*[NLS·ImpA·ImpB·NPC(n)]-[Entry NLS·ImpA·ImpB·NPC from (n)].kr*[NLS·ImpA·ImpB·NPC])*C
[Entry Ran·GTP·ImpB·NPC from (c)] = ([Entry Ran·GTP·ImpB·NPC from (c)].kf*[Ran·GTP·ImpB·NPC(c)]-[Entry Ran·GTP·ImpB·NPC from (c)].kr*[Ran·GTP·ImpB·NPC])*C
[Entry Ran·GTP·ImpB·NPC from (n)] = ([Entry Ran·GTP·ImpB·NPC from (n)].kf*[Ran·GTP·ImpB·NPC(n)]-[Entry Ran·GTP·ImpB·NPC from (n)].kr*[Ran·GTP·ImpB·NPC])*C
[Entry ImpA·CAS·Ran·GTP·NPC from (c)] = ([Entry ImpA·CAS·Ran·GTP·NPC from (c)].kf*[ImpA·CAS·Ran·GTP·NPC(c)]-[Entry ImpA·CAS·Ran·GTP·NPC from (c)].kr*[ImpA·CAS·Ran·GTP·NPC])*C
[Entry ImpA·CAS·Ran·GTP·NPC from (n)] = ([Entry ImpA·CAS·Ran·GTP·NPC from (n)].kf*[ImpA·CAS·Ran·GTP·NPC(n)]-[Entry ImpA·CAS·Ran·GTP·NPC from (n)].kr*[ImpA·CAS·Ran·GTP·NPC])*C
[Entry CAS·NPC from (c)] = ([Entry CAS·NPC from (c)].kf*[CAS·NPC(c)]-[Entry CAS·NPC from (c)].kr*[CAS·NPC])*C
[Entry CAS·NPC from (n)] = ([Entry CAS·NPC from (n)].kf*[CAS·NPC(n)]-[Entry CAS·NPC from (n)].kr*[CAS·NPC])*C

Parameter Values:
k_ImpB_on = 0.001
k_ImpB_off = 0.0001
r7.kf = 0.49
r7.kr = 0.0025
r9.kf = 0.49
r9.kr = 0.0025
r10.kf = 0.15
r10.kr = 0.00044
r11.kf = 0.001
r11.kr = 0.0001
r12.kf = 0.15
r12.kr = 0.00044
knockoff.kf = 0.02
[hydrolysis of Ran·GTP·ImpB·NPC].kf = 0.1
[CAS with NPC(c)].kf = 0.001
[CAS with NPC(c)].kr = 0.001
[CAS with NPC(n)].kf = 0.001
[CAS with NPC(n)].kr = 0.001
[CAS with Ran].kf = 0.01
[CAS with Ran].kr = 0.015
[Complex CAS with NPC(n)].kf = 0.001
[Complex CAS with NPC(n)].kr = 0.0001
[hydrolysis of ImpA·CAS·Ran·GTP·NPC].kf = 0.1
[CAS·Ran with ImpA].kf = 0.1
[CAS·Ran with ImpA].kr = 0.0001
k_pump = 0.01
[Complex CAS with NPC(c)].kf = 0.001
[Complex CAS with NPC(c)].kr = 0.0001
[Replenish NLS].kf = 0
r12_1.kf = 0.15
r12_1.kr = 0.00044
r10_1.kf = 0.15
r10_1.kr = 0.00044
r11_1.kf = 0.001
r11_1.kr = 0.0001
k_diff = 0
[Entry ImpA·ImpB·NPC from (c)].kf = 100
[Entry ImpA·ImpB·NPC from (c)].kr = 100
[ImpA·ImpB·NPC from (n)].kf = 100
[ImpA·ImpB·NPC from (n)].kr = 100
[Entry ImpB·NPC from (c)].kf = 100
[Entry ImpB·NPC from (c)].kr = 100
[Entry ImpB·NPC from (n)].kf = 100
[Entry ImpB·NPC from (n)].kr = 100
[Entry NLS·ImpA·ImpB·NPC from (c)].kf = 100
[Entry NLS·ImpA·ImpB·NPC from (c)].kr = 100
[Entry NLS·ImpA·ImpB·NPC from (n)].kf = 100
[Entry NLS·ImpA·ImpB·NPC from (n)].kr = 100
[Entry Ran·GTP·ImpB·NPC from (c)].kf = 100
[Entry Ran·GTP·ImpB·NPC from (c)].kr = 100
[Entry Ran·GTP·ImpB·NPC from (n)].kf = 100
[Entry Ran·GTP·ImpB·NPC from (n)].kr = 100
[Entry ImpA·CAS·Ran·GTP·NPC from (c)].kf = 100
[Entry ImpA·CAS·Ran·GTP·NPC from (c)].kr = 100
[Entry ImpA·CAS·Ran·GTP·NPC from (n)].kf = 100
[Entry ImpA·CAS·Ran·GTP·NPC from (n)].kr = 100
[Entry CAS·NPC from (c)].kf = 100
[Entry CAS·NPC from (c)].kr = 100
[Entry CAS·NPC from (n)].kf = 100
[Entry CAS·NPC from (n)].kr = 100
C = 1

Initial Conditions:
[NPC(c)] = 0.01
[ImpB(c)] = 1
[ImpB·NPC(c)] = 0
[ImpB(n)] = 0
[ImpA(c)] = 1
[ImpA·ImpB·NPC(c)] = 0
[ImpA·ImpB(c)] = 0
[NLS(c)] = 1
[NLS·ImpA·ImpB(c)] = 0
[NLS·ImpA·ImpB·NPC(c)] = 0
[Ran·GTP(n)] = 3
[NLS(n)] = 0
[Ran·GTP·ImpB·NPC(c)] = 0
[CAS(c)] = 1
[Ran·GDP(c)] = 0
[CAS·NPC(c)] = 0
[CAS(n)] = 0
[CAS·Ran·GTP(n)] = 0
[ImpA·CAS·Ran·GTP(n)] = 0
[ImpA·CAS·Ran·GTP·NPC(c)] = 0
[ImpA(n)] = 0
[ImpA·ImpB(n)] = 0
[ImpA·CAS·Ran·GTP(c)] = 0
[NLS·ImpA·ImpB(n)] = 0
[ImpA·ImpB·NPC(n)] = 0
[ImpB·NPC(n)] = 0
[NPC(n)] = 0.01
[NLS·ImpA·ImpB·NPC(n)] = 0
[Ran·GTP·ImpB·NPC(n)] = 0
[ImpA·CAS·Ran·GTP·NPC(n)] = 0
[CAS·NPC(n)] = 0
[ImpA·ImpB·NPC] = 0
[ImpB·NPC] = 0
NPC = 1
[NLS·ImpA·ImpB·NPC] = 0
[Ran·GTP·ImpB·NPC] = 0
[ImpA·CAS·Ran·GTP·NPC] = 0
[CAS·NPC] = 0
RanGAP = 0
RanBP1 = 0


</pre>